I am very new to R. Working mostly with Seurat package to evaluate my single-cell RNAseq data. Today I wanted to update the R version and RStudio. After that I had problems using installed packages. This is my problem:
> install.packages("Seurat", dependencies = TRUE)
Installing package into ‘C:/Users/benne/AppData/Local/R/win-library/4.2’
(as ‘lib’ is unspecified)
Warning in install.packages :
dependencies ‘S4Vectors’, ‘SummarizedExperiment’, ‘SingleCellExperiment’, ‘MAST’, ‘DESeq2’, ‘BiocGenerics’, ‘GenomicRanges’, ‘GenomeInfoDb’, ‘IRanges’, ‘rtracklayer’, ‘monocle’, ‘Biobase’, ‘limma’ are not available
trying URL 'https://cran.rstudio.com/bin/windows/contrib/4.2/Seurat_4.2.0.zip'
Content type 'application/zip' length 2376157 bytes (2.3 MB)
downloaded 2.3 MB
package ‘Seurat’ successfully unpacked and MD5 sums checked
The downloaded binary packages are in
C:\Users\benne\AppData\Local\Temp\RtmpIlveV0\downloaded_packages
> library(Seurat)
Error: package or namespace load failed for ‘Seurat’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]):
there is no package called ‘spatstat.data’
I think, there is no problem with the installation of Seurat-package but I cannot make the library-function work. I found other topics that tried to solve that problem but they did not help me.
What could be the problem? With the old R/RStudio version everything worked well. After the update I had to install the RTools42 because it said I have to do that. I have never done that before, why today??
I really hope, you guys may help me. I am totally lost!!
Attached my sessionInfo():
> sessionInfo()
R version 4.2.1 (2022-06-23 ucrt)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 10 x64 (build 22000)
Matrix products: default
locale:
[1] LC_COLLATE=German_Germany.utf8 LC_CTYPE=German_Germany.utf8 LC_MONETARY=German_Germany.utf8
[4] LC_NUMERIC=C LC_TIME=German_Germany.utf8
attached base packages:
[1] stats graphics grDevices utils datasets methods base
loaded via a namespace (and not attached):
[1] httr_1.4.4 tidyr_1.2.1 viridisLite_0.4.1 jsonlite_1.8.2 splines_4.2.1
[6] leiden_0.4.3 shiny_1.7.2 sp_1.5-0 ggrepel_0.9.1 globals_0.16.1
[11] pillar_1.8.1 lattice_0.20-45 glue_1.6.2 reticulate_1.26 digest_0.6.29
[16] RColorBrewer_1.1-3 promises_1.2.0.1 colorspace_2.0-3 plyr_1.8.7 cowplot_1.1.1
[21] htmltools_0.5.3 httpuv_1.6.6 Matrix_1.5-1 pkgconfig_2.0.3 listenv_0.8.0
[26] purrr_0.3.5 xtable_1.8-4 patchwork_1.1.2 scales_1.2.1 RANN_2.6.1
[31] later_1.3.0 Rtsne_0.16 spatstat.utils_2.3-1 tibble_3.1.8 generics_0.1.3
[36] ggplot2_3.3.6 ellipsis_0.3.2 ROCR_1.0-11 pbapply_1.5-0 SeuratObject_4.1.2
[41] lazyeval_0.2.2 cli_3.4.1 survival_3.3-1 magrittr_2.0.3 mime_0.12
[46] future_1.28.0 fansi_1.0.3 parallelly_1.32.1 MASS_7.3-57 ica_1.0-3
[51] progressr_0.11.0 tools_4.2.1 fitdistrplus_1.1-8 data.table_1.14.2 lifecycle_1.0.3
[56] matrixStats_0.62.0 stringr_1.4.1 plotly_4.10.0 munsell_0.5.0 cluster_2.1.3
[61] irlba_2.3.5.1 compiler_4.2.1 rlang_1.0.6 scattermore_0.8 grid_4.2.1
[66] ggridges_0.5.4 RcppAnnoy_0.0.19 htmlwidgets_1.5.4 igraph_1.3.5 miniUI_0.1.1.1
[71] gtable_0.3.1 codetools_0.2-18 reshape2_1.4.4 R6_2.5.1 gridExtra_2.3
[76] zoo_1.8-11 dplyr_1.0.10 fastmap_1.1.0 future.apply_1.9.1 rgeos_0.5-9
[81] utf8_1.2.2 KernSmooth_2.23-20 stringi_1.7.8 parallel_4.2.1 Rcpp_1.0.9
[86] sctransform_0.3.5 vctrs_0.4.2 png_0.1-7 tidyselect_1.2.0 lmtest_0.9-40
Thank you so much!
I tried to find out what the problem could be. I had hope that the installation of RTools42 may work but that does not make it better. The error still occurs.
CodePudding user response:
I had a similar problem with my installation of R R studio Seurat today (2022/10/26). (I did not have a problem on another computer a few weeks ago). install.packages('Seurat') said that I should install RTools. I did so, and I got a similar error messages as yours with library(Seurat) . Then, I did install.packages('spatstat.data') RTools had to recompile various things (gcc), but at the end, library(Seurat) ran smoothly.
CodePudding user response:
I had the same problem today after updating to R-4.2.1 this week. I found that restarting my computer after installing RTools and then running install.packages('spatstat.data') and install.packages('spatstat.core') worked - RTools had to compile various things as the previous answer from Masa Sato said. Now library(Seurat) is loading fine!