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How to use Python high-throughput short nucleic acid sequence alignment algorithm

Time:11-27

,, can you tell me the PCR products of high-throughput sequencing result how comparative analysis, the article is for the millions of sequences, respectively, and compare the reference sequence (the only), finally it is concluded that all of the mutation type and proportion of each mutation and sorted, how do I use Python algorithm builds, can recommend a similar algorithm structure? Beginner's white, can't find can copy algorithm, hope everybody bosses, glad to thank
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