http://www.paper.edu.cn/releasepaper/content/201202-541
Objective: to build TCTP slow virus - shRNA interference, lower target cells the expression of endogenous TCTP, method: by enzyme digestion, connection, transformation, extraction, sequencing, restructuring and other building ever TCTP expression vector (pEGFP - N1-3 flag - TCTP - GFP), 293 t cell transfection screening of RNA interference targets effectively, and RNAi lentivirus packaging in great quantities, concentration, degree of determination, results: the successful build four TCTP RNAi expression plasmid and a expression plasmid; By exogenous filter to obtain an interference effect better TCTP RNAi expression plasmid, packaging of recombinant lentivirus drops after enrichment degree is 3 x 108 TU/ml, conclusion: the successful construction and filter out interference effect better TCTP RNAi expression plasmid, and packaging a high degree of recombinant lentivirus in subsequent experiments,